方浩 求是科创学者
方浩，求是科创学者“科创百人”研究员，课题组长PI
研究方向：工业微生物细胞工厂构建及其在生物化工、生物能源、生物制药等方面的应用。
邮箱：fangh@zju.edu.cn
地址：杭州市萧山区建设三路733号浙江大学杭州国际科创中心
个人简介：
2014年12月毕业于浙江大学化学工程与生物工程学院生物化工系，工学博士。毕业后入职江南大学粮食发酵工艺与技术国家工程实验室，讲师（校聘副教授）。2016年3月入选西北农林科技大学优秀青年人才计划，调入西农生命科学学院，副教授。2021年12月入选浙江大学杭州国际科创中心“科创百人”，研究员。以第一作者或通讯作者在Chemical Engineering Journal, AIChE Journal, Biotechnology for Biofuels, Energy, Renewable Energy, Bioresource Technology等期刊发表SCI论文23篇，Google Scholar总引用大于750，H-指数16。申请国内、国际专利6项。主持国家级、省部级等多项科研基金。此外，受邀为多个国际知名期刊审稿。
研究方向：
研究兴趣聚焦工业微生物细胞工厂的工程改造。以酵母、霉菌等重要工业微生物作为研究对象，在合成生物学和系统生物学指导下，通过前向工程和逆向工程系统改造工业微生物细胞工厂，使其满足生物燃料、生物化学品、食品、生物医药、饲料等工业应用情境的需要。
主持项目：
浙江大学杭州国际科创中心科创百人计划项目（2021-2027年）
国家自然科学基金青年项目（2021-2023年）
陕西省自然科学基金（青年）（2018-2019年）
中国博士后基金特别资助（2018-2020年）
陕西省博士后基金面上项目（二等资助）（2017-2019年）
西北农林科技大学基本科研业务费（2017年）
中国博士后基金面上项目（一等资助）（2017-2019年）
西北农林科技大学优秀青年人才资助（2016-2019年）
江苏省青年基金（2015-2018年）
中央高校基本科研业务费专项资金（2015-2018年）
论文发表：
Hao Fang, Chen Zhao, Chaofeng Li, Yuqi Song, Liang Yu, Xiangyang Song, Jianping Wu, Lirong Yang. Direct consolidated bioprocessing for D-glucaric acid production from lignocellulose under subcritical water pretreatment. Chemical Engineering Journal 2023, 454: 140339.
Hao Fang, Yuntao Deng, Yingjie Pan, Chaofeng Li, Liang Yu. Distributive and collaborative push-and-pull in an artificial microbial consortium for improved consolidated bioprocessing. AIChE Journal 2022, 68(11): e17844.
Chaofeng Li, Xiaofeng Lin, Xing Ling, Shuo Li, Hao Fang*. Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium. Biotechnology for Biofuels. 2021, 14: 110. (*通讯作者)
Hao Fang, Chaofeng Li, Jiajia Zhao, Chen Zhao. Trends in engineering Trichoderma reesei towards cellulase hyperproducer. Biotechnology and Bioprocess Engineering. 2021, 26: 517-528.
Jie Yang, Lu Deng, Chen Zhao*, Hao Fang*. Heterologous expression of Neurospora crassacbh1 gene in Pichia pastoris resulted in production of a neutral cellobiohydrolase I. Biotechnology Progress 2019, 35: e2795. (*通讯作者)
Hao Fang, Runze Zhao, Chaofeng Li, Chen Zhao. Simultaneous enhancement of the beta–exo synergism and exo–exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability cellulase. Microbial Cell Factories. 2019, 18: 9.
Chen Zhao, Bing Xie, Runze Zhao, Hao Fang*. Microbial oil production by Mortierella isabellina from sodium hydroxide pretreated rice straw degraded by three-stage enzymatic hydrolysis in the context of on-site cellulase production. Renewable Energy. 2019, 130: 281-289. (*通讯作者)
Chen Zhao, Shaolin Chen, Hao Fang*. Consolidated bioprocessing of lignocellulosic biomass to itaconic acid by metabolically engineering Neurospora crassa. Applied Microbiology and Biotechnology.2018, 102: 9577-9584. (*通讯作者)
Chen Zhao, Bing Xie, Runze Zhao, Shaolin Chen, Hao Fang*.Intracellular amino and nonamino organic acids profiling of Trichosporon cutaneum on rich and limited nitrogen conditions for lipid production. Biomass and Bioenergy. 2018, 118: 84-92. (*通讯作者)
Chen Zhao, Lu Deng, Hao Fang*. Mixed culture of recombinant Trichoderma reesei and Aspergillus niger for cellulase production to increase the cellulose degrading capability. Biomass and Bioenergy. 2018, 112: 93-98. (*通讯作者)
Chen Zhao, Zongsheng Zou, Jisheng Li, Honglei Jia, Johannes Liesche, Shaolin Chen, Hao Fang*. Efficient bioethanol production from sodium hydroxide pretreated corn stover and rice straw in the context of on-site cellulase production. Renewable Energy. 2018, 118: 14-24. (*通讯作者)
Chen Zhao, Lu Deng, Hao Fang*. Microbial oil production by Mortierella isabellina from corn stover under different pretreatments. RSC Advances. 2017, 7: 56239-56246. (*通讯作者)
Chen Zhao, Hao Fang*, Shaolin Chen. Single cell oil production by Trichosporon cutaneum from steam exploded corn stover and its upgradation for production of long chain α,ω-dicarboxylic acids. Biotechnology for Biofuels. 2017, 10: 202. (*通讯作者)
Chen Zhao, Yan Yang, Linfang Wei, Qinghua Guo, Hao Fang*, Shaolin Chen, Qiang Hua. Simultaneous determination of intracellular nucleotides and coenzymes in Yarrowia lipolyticaproducing lipid and lycopene by capillary zone electrophoresis.Journal of Chromatography A. 2017, 1514: 120-126. (*通讯作者)
Chen Zhao, Zongsheng Zou, Jisheng Li, Honglei Jia, Johannes Liesche, Hao Fang*, Shaolin Chen. A novel and efficient bioprocess from steam exploded corn stover to ethanol in the context of on-site cellulase production. Energy.2017, 123: 499-510. (*通讯作者)
Hao Fang, Chen Zhao, Qin Kong, et al. Comprehensive utilization and conversion of lignocellulosic biomass for the production of long chain α,ω-dicarboxylic acids. Energy. 2016, 116, 177-189.
Hao Fang, Chen Zhao, Shaolin Chen. Single cell oil production by Mortierella isabellina from steam exploded corn stover degraded by three-stage enzymatic hydrolysis in the context of on-site enzyme production. Bioresource Technology. 2016, 216, 988-995.
Hao Fang, Liming Xia. Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw. Biomass and Bioenergy. 2015, 78, 99-109.
Hao Fang, Liming Xia. Cellulase production by recombinant Trichoderma reesei and its application in enzymatic hydrolysis of agricultural residues. Fuel. 2015, 143, 211-216.
Hao Fang, Liming Xia. High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase production. Bioresource Technology. 2013, 144, 693-697.
Hao Fang, Chen Zhao, Xiang-yang Song, et al. Enhanced cellulolytic enzymes production by synergism between Trichoderma reesei RUT-C30 and Aspergillus niger NL02 and by addition of surfactants. Biotechnology and Bioprocess Engineering. 2013, 18, 390-398.
Hao Fang, Chen Zhao, Xiang-Yang Song. Optimization of enzymatic hydrolysis of steam-exploded corn stover by two approaches: Response surface methodology or using cellulase from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger NL02. Bioresource Technology. 2010, 101, 4111-4119.
专利成果：
方浩, 吴坚平, 杨立荣. 一种绿色生物制造葡糖二酸的闭路循环系统. 中国发明专利（202211298898.6）
方浩, 赵晨, 李朝风, 吴坚平, 杨立荣. 一种直接整合生物加工木质纤维素产葡糖二酸的方法. 中国发明专利（202211163383.5）
方浩, 李朝风, 邓云涛. 一种利用人工微生物提高整合生物加工效率的方法. 中国发明专利（202210044625.2）、PCT国际专利（PCT/CN2022/095508）
方浩, 鲁辰雨, 邓云涛. 里氏木霉与酿酒酵母混合发酵白酒糟生产单细胞蛋白的方法. 中国发明专利（CN113322190A）
方浩, 谢冰, 邓璐. 一种以工农业废弃物为底物发酵产重组人胰岛素原的方法. 中国发明专利（202210364996.9）
