连佳长 科创青年卓越人才
博士，浙大“百人计划”研究员
职务：合成生物所 PI 【生物质化工教育部重点实验室副主任】
研究方向：合成生物学、基因组编辑、代谢工程
邮箱:jzlian@zju.edu.cn 电话：0571-82993283
地址：杭州市萧山区建设三路733号浙江大学杭州国际科创中心
个人简介：
连佳长博士，生物质化工教育部重点实验室副主任，化学工程与生物工程学院研究员，博士生导师，入选国家级青年人才计划、浙江大学“百人计划”以及杭州国际科创中心卓越青年人才计划，浙江省杰出青年基金获得者。主要从事基于合成生物学原理和基因组编辑技术的人工细胞工厂创建研究。主持国家自然科学基金、浙江省杰出青年基金等研究项目，共发表论文40余篇，包括Nature Communications, Metabolic Engineering, ACS Synthetic Biology, Biotechnology and Bioengineering等。受邀担任BMC Biotechnology Associate Editor、《合成生物学》学科编委、Frontiers in Biotechnology and Bioengineering客座编辑。
发表论文：
1. Jiang L, Huang L, Cai J, Xu Z, Lian J* (2020) Functional Expression of Eukaryotic Cytochrome P450s in Yeast. Biotechnol Bioeng in press
2. Dong C, Jiang L, Xu SJ, Huang L, Cai J, Lian J*, Xu Z* (2020) A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae. ACS Synth Biol 9: 2252-2257
3. Liu T, Dong C, Qi M, Zhang B, Huang L, Xu Z, Lian J* (2020) Construction of a Stable and Temperature-Responsive Yeast Cell Factory for Crocetin Biosynthesis Using CRISPR-Cas9. Front Bioeng Biotechnol 8: 653
4. Li G, Lian J, Xue H, Jiang Y, Wu M, Lin J, Yang L (2020) Enzymatic Preparation of Pyruvate by a Whole-Cell Biocatalyst Coexpressing L-Lactate Oxidase and Catalase. Process Biochem in press
5. Zhou J, Lian J, Rao CV. Metabolic Engineering of Parageobacillus thermoglucosidasius for the Efficient Production of (2R, 3R)-Butanediol. Appl Microbiol Biotechnol in press
6. Li G, Lian J, Xue H, Jiang Y, Ju S, Wu M, Lin J, Yang L (2020) Biocascade Synthesis of L-Tyrosine Derivatives by Coupling a Thermophilic Tyrosine Phenol-Lyase and L-Lactate Oxidase. Eur J Org Chem 8: 1050-1054
7. Rahman I, Fang L, Wei Z, Zheng X, Lian J*, Huang L, Xu Z* (2020) Highly Efficient Soluble Expression and Purification of Recombinant Human Basic Fibroblast Growth Factor (hbFGF) by Fusion with a New Collagen-like Protein (Scl2) in Escherichia coli. Prep Biochem Biotech in press
8. Xiao F, Wang H, Shi Z, Huang Q, Huang L, Lian J*, Cai J, Xu Z* (2020) Multi-level Metabolic Engineering of Pseudomonas mutabilis ATCC31014 for Efficient Production of Biotin. Metab Eng 61: 406-415
9. Zhao G, Yao S, Rothchild KW, Liu T, Liu Y, Lian J, He HY, Ryan KS, Du YL* (2020) The Biosynthetic Gene Cluster of Pyrazomycin—AC‐Nucleoside Antibiotic with a Rare Pyrazole Moiety. ChemBioChem 21: 644-649
10. Lian J#, Schultz C#, Cao M#, HamediRad M#, Zhao H* (2019) Multi-functional Genome-wide CRISPR System for High Throughput Genotype-Phenotype Mapping. Nat Commun 10: 5794
11. HamediRad M, Chao R, Weisberg S, Lian J, Zhao H* (2019) Towards a Fully Automated Algorithm Driven Platform for Biosystems Design. Nat Commun 10: 5150
12. Liu W, Tang D, Wang H, Lian J*, Huang L, Xu Z* (2019) Combined Genome Editing and Transcriptional Repression for Metabolic Pathway Engineering in Corynebacterium glutamicum using a Catalytically Active Cas12a. Appl Microbiol Biotechnol 103: 8911-8922
13. Liu W, Tang D, Shi R, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of S-Adenosyl-L-Methionine from DL-Methionine in Metabolic Engineered Saccharomyces cerevisiae. Biotechnol Bioeng 116: 3312-3323
14. Liu W, Zhu X, Lian J, Huang L, Xu Z* (2019) Efficient Production of Glutathione with Multi-pathway Engineering in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 46: 1685-1695
15. Gu Y, Gao J, Cao M, Dong C, Lian J*, Huang L, Cai J, Xu Z* (2019) Construction of a Series of Episomal Plasmids and their Application in the Development of an Efficient CRISPR/Cas9 System in Pichia pastoris. World J Microbiol Biotechnol 35:79
16. HamediRad M, Weisberg S, Chao R, Lian J, Zhao H* (2019) Highly Efficient Single-Pot Scarless Golden Gate Assembly. ACS Synth Biol 8:1047-1054
17. Jiang R, Chen X, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of Pseudoionone with Multi‐pathway Engineering in Escherichia coli. J Appl Microbiol, 126 (6): 1751-1760
18. Wang H, Liu W, Shi F, Huang L, Lian J*, Qu L, Cai J, Xu Z* (2018) Metabolic Pathway Engineering for High-level Production of 5-Hydroxytryptophan in Escherichia coli. Metab Eng, 48: 279-287
19. Lian J, Mishra S, Zhao H* (2018) Recent Advances in Metabolic Engineering of Saccharomyces cerevisiae: New Tools and their Applications. Metab Eng, 50: 85-108
20. Schultz C, Lian J, Zhao H. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion. Methods Enzymol, 608: 265-276
21. Jiang L, Zhao J, Lian J*, Xu Z* (2018) Cell-Free Protein Synthesis Enabled Rapid Prototyping for Metabolic Engineering and Synthetic Biology. Synth Syst Biotechnol, 3: 90-96
22. Wei P, Zhuang B, Yu D, Sharipova A, Cai J, Huang L, Lian J*, Xu Z* (2018) Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes. BioMed Res Int
23. Lian J, HamediRad M, Zhao H* (2018) Advancing Metabolic Engineering of Saccharomyces cerevisiae using the CRISPR/Cas System. Biotechnol J, 13 (9): 1700601
24. HamediRad M, Lian J, Li H, Zhao H* (2018) RNAi Assisted Genome Evolution Unveils Yeast Mutants with Superior Xylose Utilization. Biotechnol Bioeng, 115: 1552-1560
25. Lian J, Bao Z, Hu S, Zhao H* (2018) Engineered CRISPR/Cas9 System for Multiplex Genome Engineering of Polyploid Industrial Yeast Strains. Biotechnol Bioeng, 115: 1630-1635
26. Lian J, HamediRad M, Hu S, Zhao H* (2017) Combinatorial Metabolic Engineering using an Orthogonal Tri-functional CRISPR System. Nat Commun 8 (1), 1688
27. Si T#, Lian J#, Zhao H* (2017) Strain Development by Whole-Cell Directed Evolution. Directed Enzyme Evolution: Advances and Applications (Alcalde M, Ed) pp:173–200, Springer International Publishing (#Equal Contribution)
28. Lian J, Jin R, Zhao H* (2016) Construction of Plasmids with Tunable Copy Numbers in Saccharomyces cerevisiae and their Applications in Pathway Optimization and Multiplex Genome Integration. Biotechnol Bioeng 113: 2462–2473
29. Lian J, Zhao H* (2016) Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering. ACS Synt Biol 5: 689–697
30. Coursolle D, Lian J, Zhao H* (2015) Production of Long Chain Alcohols and Alkanes Upon Coexpression of an Acyl-ACP Reductase and Aldehyde-Deformylating Oxygenase with a Bacterial Type-I Fatty Acid Synthase in E. coli. Mol BioSyst 11: 2464-2472
31. Lian J, Zhao H* (2015) Recent Advances in Biosynthesis of Fatty Acids Derived Products in Saccharomyces cerevisiae via Enhanced Supply of Precursor Metabolites. J Ind Microbiol Biotechnol 42: 437-451
32. Lian J, Zhao H* (2015) Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals. ACS Synt Biol 4: 332-341
33. Feng X, Lian J, Zhao H* (2015) Metabolic Engineering of Saccharomyces cerevisiae to Improve 1-Hexadecanol Production. Metab Eng 27: 10-19
34. Lian J, Si T, Nair NU, Zhao H* (2014) Design and Construction of Acetyl-CoA Overproducing Saccharomyces cerevisiae Strains. Metab Eng 24: 139-149
35. Lian J, Li Y, Hamedi Rad H, Zhao H* (2014) Directed Evolution of a Cellodextrin Transporter for Improved Biofuel Production under Anaerobic Conditions in Saccharomyces cerevisiae. Biotechnol Bioeng 111: 1521-1531
36. Lian J, Chao R, Zhao H* (2014) Metabolic Engineering of a Saccharomyces cerevisiae Strain Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure (2R,3R)-Butanediol. Metab Eng 23: 92-99
37. Zhang X, Lian J, Kai L, Huang L, Cen P, Xu Z* (2014) Enhanced Functional Expression of Aquaporin Z via Fusion of in situ Cleavable Leader Peptides in Escherichia coli Cell-free System. Enyme Microb Technol 55: 26-30
38. Eriksen D#, Lian J#, Zhao H* (2014) Protein Design for Pathway Engineering. J Struct Biol 185(2): 234-242 (#Equal Contribution)
39. Du J, Yuan Y, Si T, Lian J, Zhao H* (2012) Customized Optimization of Metabolic Pathways by Combinatorial Transcriptional Engineering. Nucleic Acids Res 40(18): e142
40. Kai L, Kaldenhoff R, Lian J, Zhu X, Dotsch V, Bernhard F, Cen P, Xu Z* (2010) Preparative Scale Production of Functional Mouse Aquaporin 4 Using Different Cell-free Expression Modes. PLoS ONE 5(9): e12972
41. Zhou J, Huang L, Lian J, Sheng J, Cai J, Xu Z* (2010) Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system. Biotechnol Lett 32:1481-1486
42. Zhang D, Wei P, Fan L, Lian J, Huang L, Cai J, Xu Z* (2010) High-level Soluble Expression of hIGF-1 Fusion Protein in Recombinant Escherichia coli. Process Biochem 45(8): 1401-1405
43. Xu Z*, Lian J, Cai J (2010) Efficient Expression of Aquaporin Z in Escherichia coli Cell-free System Using Different Fusion Vectors. Protein Pept Lett 17(2): 181-185
44. Lian J, Ma Y, Cai J, Wu M, Wang J, Wang X, Xu Z* (2009) High-level Expression of Soluble Subunit b of F1F0-ATP Synthase in Escherichia coli Cell-free System. Appl Microbiol Biotechnol 85(2): 303-311
45. Lian J, Ding S, Cai J, Zhang D, Xu Z*, Wang X (2009) Improving Aquaporin Z Expression in Escherichia coli by Fusion Partners and Subsequent Condition Optimization. Appl Microbiol Biotechnol 82(3): 463-470
46. Lian J, Fang X, Cai J, Chen Q, Zheng Q, Kai L, Xu Z* (2008) Efficient Expression of Membrane-Bound Water Channel Protein (Aquaporin Z) in Escherichia coli. Protein Pept Lett 15(7): 687-691
