连佳长 科创青年卓越人才
博士，浙大“百人计划”研究员
职务：杭州国际科创中心副主任，生物质化工教育部重点实验室副主任，
生物工程研究所副所长
研究方向：合成生物学、代谢工程、基因组编辑、植物天然产物
邮箱:jzlian@zju.edu.cn 电话：0571-82993283
地址：杭州市萧山区平澜路2118号浙江大学杭州国际科创中心
个人简介：
连佳长，化学工程与生物工程学院研究员、博士生导师，入选国家级青年人才计划，浙江省杰出青年基金获得者。主要从事植物天然产物细胞工厂的设计与创建研究。主持国家重点研发计划课题、国家自然科学基金项目、浙江省杰出青年基金项目等基础研究项目以及普利制药、华恒生物、格林生物等企业技术开发项目。近五年，以通讯作者发表论文60余篇，包括Nature Synthesis, Nature Chemical Biology, Nature Communications, Cell Chemical Biology, Nucleic Acids Research, Trends in Biotechnology, Metabolic Engineering, ACS Synthetic Biology, Biotechnology and Bioengineering等。受邀担任《合成生物学》副主编，BioDesign Research、BMC Biotechnology等期刊编委，以及Nature Chemical Biology、Nature Synthesis、Nature Communications、Nucleic Acids Research等期刊审稿人。
发表论文：
1. Sun Y#, Chen C#, Lin C, Zhang H, Lian J*, Hong B* (2025) Elucidation and de novo reconstitution of glyceollin biosynthesis. Mol Plant, 18(5):820-832
2. Gou Y, Jiang X, Lian J* (2025) A peroxisomal membrane protein hub. Nat Chem Biol, in press
3. Gou Y, Li D, Zhao M, Li M, Zhang J, Zhou Y, Xiao F, Liu G, Ding H, Sun C, Ye C, Dong C, Gao J, Gao D, Bao Z, Huang L, Xu Z, Lian J* (2024) Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast. Nat Commun, 15:5238
4. Guo J, Gao D, Lian J*, Qu Y* (2024) De novo biosynthesis of antiarrhythmic alkaloid ajmaline. Nat Commun, 15:457
5. Gao J, Zuo Y, Xiao F, Wang Y, Li D, Xu J, Ye C, Feng L, Jiang L, Liu T, Gao D, Ma B, Huang L, Xu Z, Lian J* (2023) Biosynthesis of catharanthine in engineered Pichia pastoris. Nat Synth, 2(3): 231-242
6. Jiang Y, Sun Z, Lu K, Xu J, Wu Z, Xue H, Zhu L, Li G, Feng Y, Wu M, Lin J*, Lian J*, Yang L (2023) Manipulation of sterol homeostasis for the production of 24-epi-ergosterol in industrial yeast. Nat Commun, 14:437
7. Jiang L, Huang L, Cai J, Xu Z, Lian J* (2020) Functional Expression of Eukaryotic Cytochrome P450s in Yeast. Biotechnol Bioeng, 118(3):1050-1065
8. Dong C, Jiang L, Xu SJ, Huang L, Cai J, Lian J*, Xu Z* (2020) A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae. ACS Synth Biol 9: 2252-2257
9. Liu T, Dong C, Qi M, Zhang B, Huang L, Xu Z, Lian J* (2020) Construction of a Stable and Temperature-Responsive Yeast Cell Factory for Crocetin Biosynthesis Using CRISPR-Cas9. Front Bioeng Biotechnol 8: 653
10. Li G, Lian J, Xue H, Jiang Y, Wu M, Lin J, Yang L (2020) Enzymatic Preparation of Pyruvate by a Whole-Cell Biocatalyst Coexpressing L-Lactate Oxidase and Catalase. Process Biochem, 96:113-121
11. Zhou J, Lian J, Rao CV. Metabolic Engineering of Parageobacillus thermoglucosidasius for the Efficient Production of (2R, 3R)-Butanediol. Appl Microbiol Biotechnol, 15:75
12. Li G, Lian J, Xue H, Jiang Y, Ju S, Wu M, Lin J, Yang L (2020) Biocascade Synthesis of L-Tyrosine Derivatives by Coupling a Thermophilic Tyrosine Phenol-Lyase and L-Lactate Oxidase. Eur J Org Chem 8: 1050-1054
13. Rahman I, Fang L, Wei Z, Zheng X, Lian J*, Huang L, Xu Z* (2020) Highly Efficient Soluble Expression and Purification of Recombinant Human Basic Fibroblast Growth Factor (hbFGF) by Fusion with a New Collagen-like Protein (Scl2) in Escherichia coli. Prep Biochem Biotech, 50(6):598-606
14. Xiao F, Wang H, Shi Z, Huang Q, Huang L, Lian J*, Cai J, Xu Z* (2020) Multi-level Metabolic Engineering of Pseudomonas mutabilis ATCC31014 for Efficient Production of Biotin. Metab Eng 61: 406-415
15. Zhao G, Yao S, Rothchild KW, Liu T, Liu Y, Lian J, He HY, Ryan KS, Du YL* (2020) The Biosynthetic Gene Cluster of Pyrazomycin—AC‐Nucleoside Antibiotic with a Rare Pyrazole Moiety. ChemBioChem 21: 644-649
16. Lian J#, Schultz C#, Cao M#, HamediRad M#, Zhao H* (2019) Multi-functional Genome-wide CRISPR System for High Throughput Genotype-Phenotype Mapping. Nat Commun 10: 5794
17. HamediRad M, Chao R, Weisberg S, Lian J, Zhao H* (2019) Towards a Fully Automated Algorithm Driven Platform for Biosystems Design. Nat Commun 10: 5150
18. Liu W, Tang D, Wang H, Lian J*, Huang L, Xu Z* (2019) Combined Genome Editing and Transcriptional Repression for Metabolic Pathway Engineering in Corynebacterium glutamicum using a Catalytically Active Cas12a. Appl Microbiol Biotechnol 103: 8911-8922
19. Liu W, Tang D, Shi R, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of S-Adenosyl-L-Methionine from DL-Methionine in Metabolic Engineered Saccharomyces cerevisiae. Biotechnol Bioeng 116: 3312-3323
20. Liu W, Zhu X, Lian J, Huang L, Xu Z* (2019) Efficient Production of Glutathione with Multi-pathway Engineering in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 46: 1685-1695
21. Gu Y, Gao J, Cao M, Dong C, Lian J*, Huang L, Cai J, Xu Z* (2019) Construction of a Series of Episomal Plasmids and their Application in the Development of an Efficient CRISPR/Cas9 System in Pichia pastoris. World J Microbiol Biotechnol 35:79
22. HamediRad M, Weisberg S, Chao R, Lian J, Zhao H* (2019) Highly Efficient Single-Pot Scarless Golden Gate Assembly. ACS Synth Biol 8:1047-1054
23. Jiang R, Chen X, Lian J*, Huang L, Cai J, Xu Z* (2019) Efficient Production of Pseudoionone with Multi‐pathway Engineering in Escherichia coli. J Appl Microbiol, 126 (6): 1751-1760
24. Wang H, Liu W, Shi F, Huang L, Lian J*, Qu L, Cai J, Xu Z* (2018) Metabolic Pathway Engineering for High-level Production of 5-Hydroxytryptophan in Escherichia coli. Metab Eng, 48: 279-287
25. Lian J, Mishra S, Zhao H* (2018) Recent Advances in Metabolic Engineering of Saccharomyces cerevisiae: New Tools and their Applications. Metab Eng, 50: 85-108
26. Schultz C, Lian J, Zhao H. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion. Methods Enzymol, 608: 265-276
27. Jiang L, Zhao J, Lian J*, Xu Z* (2018) Cell-Free Protein Synthesis Enabled Rapid Prototyping for Metabolic Engineering and Synthetic Biology. Synth Syst Biotechnol, 3: 90-96
28. Wei P, Zhuang B, Yu D, Sharipova A, Cai J, Huang L, Lian J*, Xu Z* (2018) Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes. BioMed Res Int
29. Lian J, HamediRad M, Zhao H* (2018) Advancing Metabolic Engineering of Saccharomyces cerevisiae using the CRISPR/Cas System. Biotechnol J, 13 (9): 1700601
30. HamediRad M, Lian J, Li H, Zhao H* (2018) RNAi Assisted Genome Evolution Unveils Yeast Mutants with Superior Xylose Utilization. Biotechnol Bioeng, 115: 1552-1560
31. Lian J, Bao Z, Hu S, Zhao H* (2018) Engineered CRISPR/Cas9 System for Multiplex Genome Engineering of Polyploid Industrial Yeast Strains. Biotechnol Bioeng, 115: 1630-1635
32. Lian J, HamediRad M, Hu S, Zhao H* (2017) Combinatorial Metabolic Engineering using an Orthogonal Tri-functional CRISPR System. Nat Commun 8 (1), 1688
33. Si T#, Lian J#, Zhao H* (2017) Strain Development by Whole-Cell Directed Evolution. Directed Enzyme Evolution: Advances and Applications (Alcalde M, Ed) pp:173–200, Springer International Publishing (#Equal Contribution)
34. Lian J, Jin R, Zhao H* (2016) Construction of Plasmids with Tunable Copy Numbers in Saccharomyces cerevisiae and their Applications in Pathway Optimization and Multiplex Genome Integration. Biotechnol Bioeng 113: 2462–2473
35. Lian J, Zhao H* (2016) Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering. ACS Synt Biol 5: 689–697
36. Coursolle D, Lian J, Zhao H* (2015) Production of Long Chain Alcohols and Alkanes Upon Coexpression of an Acyl-ACP Reductase and Aldehyde-Deformylating Oxygenase with a Bacterial Type-I Fatty Acid Synthase in E. coli. Mol BioSyst 11: 2464-2472
37. Lian J, Zhao H* (2015) Recent Advances in Biosynthesis of Fatty Acids Derived Products in Saccharomyces cerevisiae via Enhanced Supply of Precursor Metabolites. J Ind Microbiol Biotechnol 42: 437-451
38. Lian J, Zhao H* (2015) Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals. ACS Synt Biol 4: 332-341
39. Feng X, Lian J, Zhao H* (2015) Metabolic Engineering of Saccharomyces cerevisiae to Improve 1-Hexadecanol Production. Metab Eng 27: 10-19
40. Lian J, Si T, Nair NU, Zhao H* (2014) Design and Construction of Acetyl-CoA Overproducing Saccharomyces cerevisiae Strains. Metab Eng 24: 139-149
41. Lian J, Li Y, Hamedi Rad H, Zhao H* (2014) Directed Evolution of a Cellodextrin Transporter for Improved Biofuel Production under Anaerobic Conditions in Saccharomyces cerevisiae. Biotechnol Bioeng 111: 1521-1531
42. Lian J, Chao R, Zhao H* (2014) Metabolic Engineering of a Saccharomyces cerevisiae Strain Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure (2R,3R)-Butanediol. Metab Eng 23: 92-99
43. Zhang X, Lian J, Kai L, Huang L, Cen P, Xu Z* (2014) Enhanced Functional Expression of Aquaporin Z via Fusion of in situ Cleavable Leader Peptides in Escherichia coli Cell-free System. Enyme Microb Technol 55: 26-30
44. Eriksen D#, Lian J#, Zhao H* (2014) Protein Design for Pathway Engineering. J Struct Biol 185(2): 234-242 (#Equal Contribution)
45. Du J, Yuan Y, Si T, Lian J, Zhao H* (2012) Customized Optimization of Metabolic Pathways by Combinatorial Transcriptional Engineering. Nucleic Acids Res 40(18): e142
46. Kai L, Kaldenhoff R, Lian J, Zhu X, Dotsch V, Bernhard F, Cen P, Xu Z* (2010) Preparative Scale Production of Functional Mouse Aquaporin 4 Using Different Cell-free Expression Modes. PLoS ONE 5(9): e12972
47. Zhou J, Huang L, Lian J, Sheng J, Cai J, Xu Z* (2010) Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system. Biotechnol Lett 32:1481-1486
48. Zhang D, Wei P, Fan L, Lian J, Huang L, Cai J, Xu Z* (2010) High-level Soluble Expression of hIGF-1 Fusion Protein in Recombinant Escherichia coli. Process Biochem 45(8): 1401-1405
49. Xu Z*, Lian J, Cai J (2010) Efficient Expression of Aquaporin Z in Escherichia coli Cell-free System Using Different Fusion Vectors. Protein Pept Lett 17(2): 181-185
50. Lian J, Ma Y, Cai J, Wu M, Wang J, Wang X, Xu Z* (2009) High-level Expression of Soluble Subunit b of F1F0-ATP Synthase in Escherichia coli Cell-free System. Appl Microbiol Biotechnol 85(2): 303-311
51. Lian J, Ding S, Cai J, Zhang D, Xu Z*, Wang X (2009) Improving Aquaporin Z Expression in Escherichia coli by Fusion Partners and Subsequent Condition Optimization. Appl Microbiol Biotechnol 82(3): 463-470
52. Lian J, Fang X, Cai J, Chen Q, Zheng Q, Kai L, Xu Z* (2008) Efficient Expression of Membrane-Bound Water Channel Protein (Aquaporin Z) in Escherichia coli. Protein Pept Lett 15(7): 687-691
